TnT T7 Grasp Mix (25 l) was mixed with 2 l of TnT Reaction Buffer, 1 l of TnT RNA Polymerase, 2 l of Complete Amino Acid Mix (L4461, Promega), 1 l of RNasin Ribonuclease Inhibitor (N2511, Promega), 2 l of DNA plasmid, and 17 l of nuclease-free water. with stronger TAD boundaries in mESCs. … Continue reading TnT T7 Grasp Mix (25 l) was mixed with 2 l of TnT Reaction Buffer, 1 l of TnT RNA Polymerase, 2 l of Complete Amino Acid Mix (L4461, Promega), 1 l of RNasin Ribonuclease Inhibitor (N2511, Promega), 2 l of DNA plasmid, and 17 l of nuclease-free water